Cosmid Vector for Transforming Plant and Use Thereof

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United States of America Patent

SERIAL NO

13629501

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Abstract

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The present invention provides novel cosmid vectors for plant transformation. The cosmid vectors have a full length of 15 kb or less and contain: 1) an origin of replication of an IncP plasmid, but not any origin of replication of other plasmid groups; 2) the trfA1 gene of an IncP plasmid; 3) an oriT of an IncP plasmid; 4) the incC1 gene of an IncP plasmid; 5) a cos site of lambda phage, which is located outside the T-DNA; 6) a drug resistance gene expressed in E. coli and a bacterium of Agrobacterium; 7) a T-DNA right border sequence of a bacterium of Agrobacterium; 8) a T-DNA left border sequence of a bacterium of Agrobacterium; 9) a selectable marker gene for plant transformation located between 7) and 8) and expressed in a plant; and 10) restriction endonuclease recognition site(s) located between 7) and 8) for cloning a foreign gene.

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JAPAN TOBACCO INCTOKYO JAPAN TOKYO METROPOLIS

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
HIEI, Yukoh Iwata-shi, Shizuoka, JP 25 331
IMAYAMA, Teruyuki Iwata-shi, Shizuoka, JP 5 10
ISHIDA, Yuji Iwata-shi, Shizuoka, JP 50 590
KOMARI, Toshihiko Iwata-shi, Shizuoka, JP 15 512
KOMORI, Toshiyuki Iwata-shi, Shizuoka, JP 13 50
MINE, Toshiki Iwata-shi, Shizuoka, JP 10 93
TAKAKURA, Yoshimitsu Iwata-shi, Shizuoka, JP 44 149

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