Cell culture medium
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United States of America Patent
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Mar 24, 2015
Grant Date -
Apr 25, 2013
app pub date -
Apr 25, 2011
filing date -
Apr 26, 2010
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Abstract
A cell culture medium with high content of choline chloride is provided. The cell culture media further comprise only moderate amounts of amino acids, in particular the amount of glutamine in the cell culture media is limited. The cell culture media can be used for large scale production of polypeptides using cell cultures. The cell culture media with high content of choline chloride are particularly suitable for fed-batch cell culture whereby cell viabilities stay at a higher level for a longer time and high polypeptide titers although limited amounts of amino acids are used.
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