Polypeptide producing cells
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United States of America Patent
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Sep 24, 2013
Grant Date -
May 2, 2013
app pub date -
Dec 5, 2012
filing date -
May 17, 2006
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Abstract
The current invention describes a nucleic acid comprising in a 5′ to 3′ direction a) a first nucleic acid encoding a heterologous polypeptide without an in frame stop codon, b) a second nucleic acid beginning with a 5′ splice donor site and terminated by a 3′ splice acceptor site comprising an in frame translational stop codon and a polyadenylation signal, and c) a nucleic acid encoding i) at least a fragment of a transmembrane domain, or ii) a signal peptide for a GPI-anchor.
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