NOVEL ENZYME PROTEIN, PROCESS FOR PRODUCTION OF THE ENZYME PROTEIN THE SAME, AND GENE ENCODING THE ENZYME PROTEIN THE SAME
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United States of America Patent
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Issued Date -
May 16, 2013
app pub date -
Jul 28, 2011
filing date -
Jul 30, 2010
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Abandoned
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Abstract
The present invention relates to a protein having a β-galactoside-α2,3-sialyltransferase activity but substantially not having any neuraminidase activity, a nucleic acid encoding the enzyme protein, and a method of producing the enzyme using a microorganism transformed with a gene encoding the protein. The protein of the present invention is a modified-type enzyme protein produced by mutating a specific amino acid residue in a β-galactoside-α2,3-sialyltransferase protein derived from a microorganism belonging to the genus Photobacterium of the family Vibrionaceae and thereby substantially inactivating the neuraminidase activity of the protein.
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