Method and device for identifying multipotent stem cell colony, and method and device for automatically culturing multipotent stem cells
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United States of America Patent
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Mar 10, 2015
Grant Date -
May 23, 2013
app pub date -
Jun 22, 2011
filing date -
Jun 25, 2010
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Abstract
A method and device achieves identification on whether a colony on a cultivation container is differentiated or undifferentiated on an occasion of cultivation of multipotent stem cell, and a method and device achieves a rapid culture of undifferentiated multipotent stem cells. Undifferentiated colony containing only undifferentiated multipotent stem cells is identified from other types of colonies by means of the circularity C of the colony in an image-processed taken image of the inside of cultivation container of a multipotent stem cell. More specifically, when a first threshold of the circularity of the colony is C1, and a second predetermined threshold is C2 (C1
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