Method of synthesizing a suppressor tRNA, DNA construct and use thereof for producing a non-natural amino acid-incorporated protein

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United States of America Patent

PATENT NO 8822180
APP PUB NO 20130130313A1
SERIAL NO

13460773

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Abstract

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There are provided a DNA construct comprising a suppressor tRNA gene of a non-eukaryote containing no internal promoter functioning in a eukaryotic cell, and a eukaryotic or bacteriophage promoter linked at the 5′ end of the tRNA gene, a method for synthesizing a suppressor tRNA by using the DNA construct, and a process for producing protein incorporating a non-natural amino acid by using the same.

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RIKENTOKYO JAPAN

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hino, Nobumasa Yokohama, JP 2 15
Kobayashi, Takatsugu Yokohama, JP 9 30
Mukai, Takahito Yokohama, JP 9 89
Sakamoto, Kensaku Yokohama, JP 18 102
Yokoyama, Shigeyuki Yokohama, JP 81 779

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