HIGH THROUGHPUT SINGLE NUCLEOTIDE POLYMORPHISM ASSAY
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United States of America Patent
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Issued Date -
May 30, 2013
app pub date -
Nov 27, 2012
filing date -
Nov 29, 2011
priority date (Note) -
Abandoned
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Abstract
A method consisting of a homogeneous assay detection system for a PCR process using FRET for detection and zygosity analysis of the HaAHASL1-A122(At)T single nucleotide polymorphism in sunflower is provided. The method provides specific sunflower-genome primers that can be used to detect the presence or absence of the HaAHASL1-A122(At)T single nucleotide polymorphism. The primer combinations for use in an endpoint PCR assay capable of determining zygosity and for assisting in breeding introgression are described.
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