METHODS AND COMPOSITIONS FOR PREPARING SAMPLES FOR IMMUNOSTAINING
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United States of America Patent
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Issued Date -
Jun 27, 2013
app pub date -
Nov 2, 2012
filing date -
Nov 3, 2011
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Abstract
Compositions and methods for preparing a sample for immunological staining are provided. Compositions include kits comprising a first solution comprising a surfactant and a second solution comprising a chaotropic agent. Methods comprise contacting a sample, such as cells or tissues, with a first solution comprising a surfactant and then contacting the sample with a second solution comprising a chaotropic agent. The method does not require extreme heat for antigen retrieval and therefore, maintains the cellular morphology of the sample.
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