Sequence Specific Real-Time Monitoring of Loop-Mediated Isothermal Amplification (LAMP)

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United States of America Patent

APP PUB NO 20130171643A1
SERIAL NO

13805578

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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Gene-based diagnostics capable of rapidly discriminating selected strains of a selected pathogen from other populations within the same species are disclosed. Sequence-specific, real-time monitoring of LAMP of DNA may be accomplished through the use of oglionucleotide probes, referred to as “assimilating probes.” The assimilating probes include two oglionucleotide strands, one which includes a quencher (referred to as the quenching probe) and another which includes a fluorophore (referred to as the fluorescent probe). A fluorescent signal results when the two strands are displaced from one another during the LAMP reaction. By monitoring the emitted fluorescence, sequence specific amplification may be detected.

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Patent Owner(s)

Patent OwnerAddress
UNIVERSITY OF HAWAII2425 CAMPUS ROAD SINCLAIR 10 HONOLULU HI 96822

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Jenkins, Daniel M Honolulu, US 6 39
Kubota, Ryo Honolulu, US 118 1165

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