METHOD FOR IMPROVING CLEAVAGE OF DNA BY ENDONUCLEASE SENSITIVE TO METHYLATION
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United States of America Patent
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Issued Date -
Aug 1, 2013
app pub date -
Jun 15, 2011
filing date -
Jun 15, 2010
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Abandoned
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Abstract
The present invention concerns novel methods for improving cleavage of DNA by rare-cutting endonucleases, overcoming DNA modification constraints, particularly DNA methylation, thereby giving new tools for genome engineering, particularly to increase the integration efficiency of a transgene into a genome at a predetermined location, including therapeutic applications and cell line engineering.
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