INDUCED SPORULATION SCREENING METHOD
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United States of America Patent
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Issued Date -
Aug 29, 2013
app pub date -
Aug 18, 2011
filing date -
Aug 19, 2010
priority date (Note) -
Abandoned
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Abstract
The present invention relates to a method of screening for an improved enzyme variant, said method comprising the steps of: (i) providing a recombinant host cell capable of sporulating comprising a polynucleotide encoding a sporulation factor and a polynucleotide encoding an enzyme variant which are operably linked to an inducible promoter; (ii) culturing the host cell under conditions suitable to induce the formation of spores; (iii) culturing the spores obtained in step (ii) in a medium containing a substrate for the enzyme variant; and (iv) determining the activity of the enzyme variant.
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