Method of differentiating stem cells
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United States of America Patent
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Jul 18, 2017
Grant Date -
Aug 29, 2013
app pub date -
Mar 14, 2013
filing date -
Oct 13, 2009
priority date (Note) -
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Abstract
The present disclosure provides methods of generating germ layers from stem cells comprising culturing the stem cells in a culture media having osmolality ranges that promote the generation of specific germ layer progenitor cells. The present disclosure also includes a method to generate different cell lineages from the germ layers as well as to detect them by immunological methods. The present disclosure further provides methods for the generation, isolation, cultivation and propagation of committed progenitor cells and for the production of differentiated cells from the three germ layers. The present disclosure also provides culture media and kits for use in inducing the three germ layers.
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