Gram staining method with improved decolorization of the crystal violet-iodine complex from gram negative bacteria

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United States of America Patent

PATENT NO 9023615
APP PUB NO 20130244252A1
SERIAL NO

13773864

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Abstract

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Provided herein are methods of staining biological material for the purpose of detecting, and in some examples also identifying, microorganisms. Methods of Gram staining bacteria using a slow-acting decolorizing formulation, such as one that includes 1,2-propandiol or ethylene glycol, can be used to extend the time of the decolorizing step, and thus permit automation of the Gram staining method. Also provided are compositions and kits for performing automated Gram staining on microscope slides.

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Patent Owner(s)

  • VENTANA MEDICAL SYSTEMS, INC.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Connolly, Cynthia Tucson, US 2 7
Duquette, Setareh Oro Valley, US 6 2
Mehta, Parula Oro Valley, US 14 299
Schumack, Lesley Tucson, US 3 1
Weidner, Charles Tucson, US 4 9

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