METHODS AND APPARATUS FOR AMPLIFYING NUCLEIC ACIDS
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United States of America Patent
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Issued Date -
Sep 26, 2013
app pub date -
Sep 21, 2011
filing date -
Sep 21, 2010
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Abstract
A method of amplifying nucleic acid includes providing a microfluidic device having a space therein. The space is filled with a gel medium. Reagents for carrying out a PCR reaction are supported within the matrix of the gel medium within the space. A nucleic acid containing sample is brought into contact with the gel medium. The sample having whole cells or cell lysate without any prior separation of the nucleic acid from other components of the cells. PCR amplification of nucleic acid from the sample is performed in the space using the PCR reagents.
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