METHOD FOR PRODUCING A POLYPEPTIDE IN YARROWIA LIPOLYTICA
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United States of America Patent
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Issued Date -
Nov 7, 2013
app pub date -
May 26, 2011
filing date -
May 28, 2010
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Abandoned
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Abstract
This invention relates to a method for the production of proteins, preferably, heterologous proteins, under the regulation of the hp4d promoter, in Yarrowia lipolytica. In particular, this invention describes a method for manipulating the growth rate of Y. lipolytica by regulating the carbon and/or nitrogen supply. A growth rate of less than 0.045 h″l was found to be optimal for increasing Y. lipolytica biomass and increasing the amount of heterologous proteins of interest produced.
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