METHODS AND MATERIALS USING SIGNALING PROBES
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United States of America Patent
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Issued Date -
Dec 5, 2013
app pub date -
Mar 15, 2013
filing date -
Feb 18, 2004
priority date (Note) -
Abandoned
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Abstract
The present invention relates to methods of isolating cells, generating cell lines, and detecting RNAs in cells using signaling probes that produce a signal upon hybridization to a target sequence. Other methods that utilize the signaling probe include methods of detecting or quantifying the effect of an agent on RNAs in a cell, methods of quantifying the level of RNA expression, methods for identifying genetic recombinational events in living cells and methods of generating a transgenic animal using the isolated cells. The invention also provides protease probes. Signaling probes and protease probes that form stem-loop structures, three-arm junction structures, and dumbbell structures are provided.
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