ENRICHMENT AND CONCENTRATION OF SELECT PRODUCT ISOFORMS BY OVERLOADED BIND AND ELUTE CHROMATOGRAPHY
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United States of America Patent
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Issued Date -
Dec 12, 2013
app pub date -
Nov 15, 2011
filing date -
Nov 15, 2010
priority date (Note) -
Abandoned
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Abstract
Disclosed is a method for enhancing or increasing the concentration of biological product in a final mixture, wherein said biological product has one or more selected characteristics, wherein said method comprises: (a) allowing an initial mixture of biological products with and without said selected characteristics to contact a chromatography medium wherein the quantity of biological products in said initial mixture exceeds the binding capacity or the dynamic binding capacity of said chromatography medium; (b) allowing biological product not having said one or more selected characteristics to be separated by said chromatography medium; and (c) recovering a final mixture of biological products from said chromatography medium wherein said final mixture comprises an enhanced or increased concentration of biological product with one or more selected characteristics, compared to the concentration of biological product in said initial mixture.
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