Nuclease Reduction
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United States of America Patent
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Issued Date -
Jan 9, 2014
app pub date -
Sep 11, 2013
filing date -
Aug 20, 2007
priority date (Note) -
Abandoned
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Abstract
A method for reducing nuclease activity in a subtilisin solution obtained from a fermentation broth comprising
- a) adjusting the pH of the subtilisin solution to a pH in the range of from pH 8.5 to pH 10.5;b) adding a polyol;c) adjusting the temperature of the subtilisin solution to a temperature in the range of from 50° C. to 80° C.;whereby the nuclease activity is reduced to less than 5% of the initial value and the subtilisin activity is maintained at more than 80% of the initial value.
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