CELL TREATMENT SOLUTION AND METHOD OF PREPARING STAINED CELL SUSPENSION FOR A MEASUREMENT OF NUCLEAR DNA BY FLOW CYTOMETRY
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United States of America Patent
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app pub date -
Apr 25, 2013
filing date -
Feb 9, 2009
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Abstract
A cell treatment solution and a method that is used for preparing a stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry. The cell treatment solution may include a surfactant, RNase, and a fluorescent dye. The surfactant may include, for example, a non-ionic surfactant, a zwitterionic surfactant, an anionic surfactant, and/or a cationic surfactant. In one method of the presently disclosed subject matter, stained cell suspension that is provided to a measurement of nuclear DNA by flow cytometry is prepared. The method may include adding a tissue sample to a cell treatment solution including a surfactant, RNase, and fluorescent dye, disaggregating the tissue sample, and filtering the disaggregated tissue sample. Another method of the presently disclosed subject matter includes disaggregating a tissue sample, preparing cell suspension by filtering the disaggregated tissue sample, and adding a cell treatment solution including a surfactant, RNase, and fluorescent dye.
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