ENGINEERED INTRACELLULAR SIALYLATION PATHWAYS
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United States of America Patent
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Issued Date -
Jan 23, 2014
app pub date -
Aug 27, 2013
filing date -
Mar 2, 1999
priority date (Note) -
Abandoned
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Abstract
Methods for manipulating carbohydrate processing pathways in cells of interest are provided. Methods are directed at manipulating multiple pathways involved with the sialylation reaction by using recombinant DNA technology and substrate feeding approaches to enable the production of sialylated glycoproteins in cells of interest. These carbohydrate engineering efforts encompass the implementation of new carbohydrate bioassays, the examination of a selection of insect cell lines and the use of bioinformatics to identify gene sequences for critical processing enzymes. The compositions comprise cells of interest producing sialylated glycoproteins. The methods and compositions are useful for heterologous expression of glycoproteins.
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