PROCESSING POLYNUCLEOTIDE-CONTAINING SAMPLES
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United States of America Patent
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Issued Date -
Jan 30, 2014
app pub date -
Jun 24, 2013
filing date -
May 3, 2004
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Abstract
Methods and systems for processing polynucleotides (e.g., DNA) are disclosed. A processing region includes one or more surfaces (e.g., particle surfaces) modified with ligands that regain polynucleotides under a first set of conditions (e.g., temperature and pH) and release the polynucleotides under a second set of conditions (e.g., higher temperature and/or more basic pH). The processing region can be used to, for example, concentrate polynucleotides of a sample and/or separate inhibitors of amplification reactions from the polynucleotides. Microfluidic devices with a processing region are disclosed.
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