NONADHESIVE SPHERE CELL CULTURE CONTAINER, SYSTEM, AND METHOD
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United States of America Patent
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Issued Date -
Feb 6, 2014
app pub date -
Dec 13, 2012
filing date -
Jul 31, 2012
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Abandoned
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Abstract
The present invention provides a nonadhesive sphere cell culture container, system and method, which is performed by coating a gel film on the bottom of the culture container, and then by immersing the liquid medium containing cells on the gel film to cultivate cells as the sphere cells. Abundant cancer stem cells with sphere formation can be harvested by the invention with advantages of marked reduction of cost, time-saving (takes only 7-10 days for spheres formation), and without additional separation technique.
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