PRIMER AND METHOD FOR QUANTITATIVE ASSAY OF MICRORNA AND APPLICATION OF SAME
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United States of America Patent
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Oct 17, 2013
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Apr 20, 2011
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Abstract
A method and primer for quantitative assay of microRNAs (miRNAs) and application of the same are provided. In this method, miRNAs are subjected to polyadenylation and subsequent reverse transcription with miRNA-specific S-Oligo(dT) RT primers. The miRNA is then assayed in a real-time PCR quantitative assay using a miRNA-specific forward primer, a universal reverse primer and a universal probe. The methods in the present invention have improved sensitivity, specificity, and efficiency in miRNA quantification assay, providing a simple, high-throughput, and cost-effective tool applicable for the early diagnosis and prognosis of many severe diseases such as malignancy.
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