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Abstract

Primers suitable for nucleic acid sequence amplification of Pongamia plant genetic markers and a method of genetic analysis of Pongamia plants are provided. The primers comprise a repeat unit of two or three nucleotides repeated five to ten times together with a three prime extension of two or three nucleotides. Genetic markers amplified by the primers are also provided, from which may be produced further primers for genetic analysis of Pongamia plants. The primers, genetic markers and methods of genetic analysis may be suitable for selection and breeding of Pongamia plants having desired traits such as, or relating to, seed size, seed number, seed oil content, seed oil quality, seed flavour and toxicity, disease resistance, water use efficiency, nitrogen use efficiency, precocious flowering, flowering time, tree size, tree shape, growth rate, drought tolerance, salinity tolerance and/or growth in low-nutrient soils.

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