Recombinant Beta-Glucosidase Variants for Production of Soluble Sugars from Cellulosic Biomass
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United States of America Patent
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Issued Date -
Mar 6, 2014
app pub date -
Nov 8, 2013
filing date -
Nov 25, 2009
priority date (Note) -
Abandoned
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Abstract
The invention relates to recombinant expression of a variant form of a fungal C1 strain β-glucosidase. The invention also relates to the generation of fermentable sugars from biomass and the production of biofuels by fermentation of the sugars using genetically modified organisms expressing the β-glucosidase variant. The invention provides methods for producing a fermentable sugar, such as glucose, from cellobiose by contacting cellobiose with a recombinant β-glucosidase variant protein, such as a variant protein secreted by a recombinant host cell into culture medium. Methods of the invention may be used for conversion of a biomass substrate to a fermentable sugar, and ultimately to ethanol or other biofuel.
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