Synthetic luciferase gene and protein
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United States of America Patent
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Issued Date -
Mar 27, 2014
app pub date -
Dec 4, 2012
filing date -
Oct 5, 2010
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Abandoned
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Abstract
A codon optimized and stabilized luciferase gene and a novel recombinant DNA characterized by incorporating this new gene coding for a novel luciferase into a vector DNA for improved activities in mammalian cells, are disclosed. This new luciferase exhibits long-wavelength light emission, as well as improved thermostability and higher expression levels in mammalian cell systems, compared to native luciferase. Assays using this new enzyme for measuring various biological metabolic functions are described.
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