Automatic threshold setting and baseline determination for real-time PCR

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United States of America Patent

PATENT NO 10002229
SERIAL NO

14047314

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Abstract

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The invention discloses a system and methods for quantitating the presence of nucleic acid sequences by evaluation of amplification data generated using real-time PCR. In one aspect, the methods may be adapted to identify a threshold and threshold cycle for one or more reactions based upon evaluation of exponential and baseline regions for each amplification reaction. The methodology used in the analysis may be readily automated such that subjective user interpretation of the data is substantially reduced or eliminated.

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Patent Owner(s)

  • APPLIED BIOSYSTEMS, LLC

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Abbasi, Nasser M San Mateo, US 7 48
Lewis, Clinton Palo Alto, US 8 56
Woo, David C Foster City, US 9 49

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