Detection method uses magnetic and detectable nanoparticles with oligonucleotides attached thereto
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United States of America Patent
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Dec 6, 2016
Grant Date -
May 8, 2014
app pub date -
Nov 6, 2012
filing date -
Nov 6, 2012
priority date (Note) -
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Abstract
A method and kit for determining a nucleic acid is provided, including: providing magnetic nanoparticles and detectable nanoparticles to the sample, wherein the magnetic nanoparticles and detectable nanoparticles respectively contain oligonucleotides attached thereto, and the detectable nanoparticles contain at least one kind of nanoparticles with detectable signals distinct from the others, and the oligonucleotides attached on each kind of the detectable nanoparticles are complementary to a region of one of the nucleic acids in the sample; reacting the magnetic and detectable nanoparticles with the sample; and detecting signals from each kind of the detectable nanoparticles for determining the nucleic acid for each in the sample.
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