METHODS FOR POLYMERASE CHAIN REACTION COPY NUMBER VARIATION ASSAYS
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United States of America Patent
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Issued Date -
Jun 12, 2014
app pub date -
Dec 5, 2013
filing date -
Dec 5, 2012
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Abandoned
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Abstract
This disclosure provides methods for measuring the copy number for highly amplified and/or abundant genomic loci. Recognized herein is a need for methods for determining nucleic acid copy number, particularly in instances where one locus to be quantified (i.e., the target) is relatively more abundant than a locus of known abundance (i.e., the reference). In some cases, the method involves combining a query nucleic acid sample with a diluting nucleic acid sample and measuring the relative copy number of a target sequence compared with a reference sequence in the combined sample.
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