CELL-BASED METHOD TO DETECT SKIN SENSITIZERS
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United States of America Patent
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Issued Date -
Jul 10, 2014
app pub date -
Jan 4, 2013
filing date -
Jan 4, 2013
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Abstract
A method of predicting whether a compound is a skin sensitizer using an in vitro approach. The method includes a step of imaging immune effector cells positioned within a plurality of containers to obtain imaged cellular targets, each container being treated with a different concentration of a compound. The method further includes a step of quantitatively measuring the imaged cellular targets over a range of concentrations of the compound to detect changes in multiple cellular targets of the immune effector cells associated with skin sensitivity. The method further includes a step of analyzing measurements obtained from the measured imaged cellular targets over the range of concentrations of the compound to determine whether the compound is a skin sensitizer.
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