METHODS FOR TRUE ISOTHERMAL STRAND DISPLACEMENT AMPLIFICATION
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United States of America Patent
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Sep 11, 2014
app pub date -
Mar 10, 2014
filing date -
Mar 11, 2013
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Abstract
Methods, primers and probes are provided for the isothermal amplification and detection, without denaturation, of double stranded nucleic acid targets for polymerase strand displacement amplification (“iSDA”). The methods and compositions disclosed are highly specific for nucleic acid targets with high sensitivity, specificity and speed that allow detection of clinical relevant target levels. The methods and compositions can easily be used to amplify or detect nucleic acid targets in biological samples.
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