METHOD OF SEQUENCING A FULL MICRORNA PROFILE FROM CEREBROSPINAL FLUID
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United States of America Patent
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Sep 18, 2014
app pub date -
Mar 15, 2014
filing date -
Mar 15, 2013
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Abstract
The present invention provides methods of purifying RNA from a biological sample comprising two separate aqueous extractions of the organic phase obtained by mixing the biological sample with a first solution comprising guanidinium isothiocyanate and beta-mercaptoethanol and a second solution comprising phenol, chloroform, and isoamyl alcohol. Also provided are methods of sequencing RNA purified from a biological sample and methods of diagnosing Alzheimer's disease and Parkinson's disease in a subject by determining whether a plurality of miRNAs has deregulated expression in biological sample from the subject.
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