Stable Genomic Integration of Multiple Polynucleotide Copies
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United States of America Patent
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Mar 20, 2014
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Oct 22, 2004
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Abstract
Methods of constructing a cell comprising in its chromosome one or more copies of an open reading frame (ORF) or operon encoding at least one polypeptide of interest, each copy being under the transcriptional control of a heterologous promoter using a site specific recombinase and in vivo integration by recombination; means for carrying out the methods, resulting cells, and methods for producing a polypeptide of interest using the resulting cells.
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