Method for producing phosphoserine incorporated proteins by using SepRS mutants and EF-Tu mutants

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United States of America Patent

PATENT NO 9322044
APP PUB NO 20140335561A1
SERIAL NO

14210486

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Abstract

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The present invention relates to a method of producing a phosphorylated protein using a SepRS (O-phosphoseryl-tRNA synthetase) mutant and an EF-Tu mutant, which have increased activity. More specifically, the invention relates to a method of producing a phosphorylated protein by incorporating phosphoserine into the specific position of a target protein or polypeptide using tRNASep serving to recognize at least one codon in the mRNA of the target protein or polypeptide, an O-phosphoseryl-tRNA synthetase (SepRS) mutant selected by a molecular evolution technique and serving to aminoacylate tRNASep with phosphoserine (Sep), and an EF-Tu mutant serving to bind and deliver Sep-tRNASep to the ribosome.

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Patent Owner(s)

  • KOREA INSTITUTE OF SCIENCE AND TECHNOLOGY

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Lee, Sangsik Daejeon, KR 3 16
Park, Hee Sung Daejeon, KR 15 9

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