METHODS FOR PRODUCING RETINAL TISSUE AND RETINA-RELATED CELL
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United States of America Patent
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Nov 20, 2014
app pub date -
Nov 22, 2012
filing date -
Nov 25, 2011
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Abstract
The invention provides a method for producing a retinal tissue by (1) subjecting pluripotent stem cells to floating culture in a serum-free medium containing a substance inhibiting the Wnt signal pathway to form an aggregate of pluripotent stem cells, (2) subjecting the aggregate to floating culture in a serum-free medium containing a basement membrane preparation, and then (3) subjecting the aggregate to floating culture in a serum-containing medium. The invention also provides a method for producing an optic-cup-like structure, a method for producing a retinal pigment epithelium, and a method for producing a retinal layer-specific neural cell.
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