EXTENDED DEPTH OF FIELD THREE-DIMENSIONAL NANO-RESOLUTION IMAGING METHOD, OPTICAL COMPONENT, AND IMAGING SYSTEM
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United States of America Patent
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Issued Date -
Nov 27, 2014
app pub date -
Jun 26, 2013
filing date -
Nov 19, 2012
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Abandoned
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Abstract
An extended depth of field three-dimensional nano-resolution imaging method includes: creating an optical module with a double helix point spread function and multi-stage imaging properties of a defocus optical grating; obtaining double helix image of a molecule by imaging a molecule using the optical module; determining a lateral position of the molecule according to a position of a midpoint of double helix sidelobes on the imaging plane in the double helix image; determining an axial position of the molecule according to a rotation angle of a line of centers of the double helix sidelobes on the imaging plane and the position of the midpoint of the double helix sidelobes on the imaging plane in the double helix image. The double helix point spread function and the defocus optical grating multi-stage imaging are combined to implement three-dimensional imaging to extended the depth of field and to improve the resolution.
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