Method of welding a frozen aqueous sample to a microprobe
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United States of America Patent
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Sep 22, 2015
Grant Date -
Dec 18, 2014
app pub date -
Jun 11, 2014
filing date -
Jun 14, 2013
priority date (Note) -
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Abstract
The invention relates to a method of welding a vitreous biological sample at a temperature below the glass transition temperature of approximately −137° C. to a micromanipulator, also kept at a temperature below the glass transition temperature. Where prior art methods used IBID with, for example, propane, or a heated needle (heated resistively or by e/g/laser), the invention uses a vibrating needle to locally melt the sample. By stopping the vibration, the sample freezes to the micromanipulator. The heat capacity of the heated parts is small, and the amount of material that stays in a vitreous condition thus large.
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