Method to Quantify siRNAs, miRNAs and Polymorphic miRNAs
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United States of America Patent
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Issued Date -
Dec 25, 2014
app pub date -
Mar 28, 2014
filing date -
Aug 24, 2005
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Abandoned
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Abstract
The present teachings provide methods, compositions, and kits for quantifying target polynucleotides. In some embodiments, a reverse stem-loop ligation probe is ligated to the 3′ end of a target polynucleotide, using a ligase that can ligate the 3′ end of RNA to the 5′ end of DNA using a DNA template, such as T4 DNA ligase. Following digestion to form an elongated target polynucleotide with a liberated end, a reverse transcription reaction can be performed, followed by a PCR. In some embodiments, the methods of the present teachings can discriminate between polymorphic polynucleotides that vary by as little as one nucleotide.
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