MALDI MASS SPECTROMETRY OF PEPTIDES

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United States of America Patent

APP PUB NO 20150001386A1
SERIAL NO

13933091

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ATTORNEY / AGENT: (SPONSORED)

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Abstract

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The present invention provides a mass spectrometry method capable of detecting a smaller amount of peptide. A method for analyzing a peptide by matrix-assisted laser desorption/ionization mass spectrometry, comprising the steps of: dropping, onto a target plate, a peptide solution using, as a solvent, an aqueous solution containing 15 to 50 vol % of acetonitrile, and a matrix solution that uses, as a solvent, an aqueous solution containing 15 to 50 vol % of acetonitrile and that contains 0.05 to 1 mg/mL α-cyano-4-hydroxycinnamic acid, respectively, to prepare a droplet of peptide-matrix mixed solution; removing the solvent from the droplet of peptide-matrix mixed solution to prepare a sample for mass spectrometry as a residue; and irradiating the sample for mass spectrometry with laser to detect the peptide.

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Patent Owner(s)

Patent OwnerAddress
SHIMADZU CORPORATION1 NISHINOKYO-KUWABARACHO NAKAGYO-KU KYOTO-SHI KYOTO 6048511

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Hioki, Yusaku Kyoto-shi, JP 5 4
Kuyama, Hiroki Kyoto-shi, JP 14 58

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