METHOD FOR SUPPRESSING THE EFFECTS OF ASCORBIC ACID
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United States of America Patent
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Issued Date -
Jan 1, 2015
app pub date -
Feb 6, 2013
filing date -
Feb 9, 2012
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Abandoned
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Abstract
Provided is a method for suppressing an effect of ascorbic acid on a measurement of glycated hemoglobin in a glycated hemoglobin-containing sample. The method of the present invention is a method for suppressing an effect of ascorbic acid on the measurement of glycated hemoglobin in a glycated hemoglobin-containing sample, comprising reacting the glycated hemoglobin-containing sample with a proteolytic enzyme, reacting a generated glycated peptide with a glycated peptide oxidase, and then measuring a generated hydrogen peroxide, wherein the reaction of the glycated peptide with the glycated peptide oxidase is performed in the presence of a halogen oxide and a substance selected from the group consisting of a substance represented by formula (I), wherein R1 represents substituted or unsubstituted alkyl and a substance represented by the general formula (II), wherein R2 represents substituted or unsubstituted alkyl; and represents a single bond or a double bond.
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