CRYOPRESERVATION OF CELLS IN ABSENCE OF VITRIFICATION INDUCING AGENTS
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United States of America Patent
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Jan 15, 2015
app pub date -
Feb 7, 2013
filing date -
Feb 8, 2012
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Abstract
The present invention relates to a method for cryopreserving biological material. In particular, the method comprises storing the biological material at a cryopreserving temperature in a composition comprising polyvinyl alcohol (PVA). wherein the composition is substantially free of vitrification-inducing agents such as DMSO and glycerol. The invention also provides methods of inhibiting ice recrystallisation and of reducing cell damage during the warming or thawing of a cryopreserved composition comprising biological material. The invention also relates to processes for producing a biological material, and related kits.
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