ASSAY FOR DETERMINING RELATIVE REDOX CHANGES IN LIVING CELLS AND ASSOCIATED DEVICES, SYSTEMS, AND METHODS
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United States of America Patent
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Jan 15, 2015
app pub date -
Apr 14, 2014
filing date -
Apr 12, 2013
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Abstract
The present disclosure provides conjugates, systems, devices, and methods for detecting cellular redox state. In one aspect, for example, a conjugate for detecting cellular redox state can include a first segment including a cell penetrating peptide conjugated to a first detection molecule, and a second segment including a cargo peptide conjugated to a second detection molecule, wherein the first segment and the second segment are coupled together by a redox-sensitive linkage, and wherein the first detection molecule and the second detection molecule have properties that allow linked proximity detection. In one specific example, the first detection molecule and the second detection molecule include fluorophore/quencher pair.
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