FRET MEASUREMENT DEVICE AND FRET MEASUREMENT METHOD
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United States of America Patent
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Issued Date -
Feb 12, 2015
app pub date -
Mar 22, 2013
filing date -
Mar 22, 2012
priority date (Note) -
Abandoned
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Abstract
FRET measurement uses a FRET probe that includes a probe element X labeled with a donor fluorescent substance and a probe element Y labeled with an acceptor fluorescent substance and enables FRET to occur when the probe element X and the probe element Y approach to each other or bind together. A test sample as a measuring object in FRET measurement contains a test object about which it is unknown whether or not it has an approaching/binding property of allowing the probe element X and the probe element Y to approach to each other or bind together or a separating property of separating from each other the probe element X and the probe element Y that are in a state where they adjoin each other or bind together. A plurality of sets of a fluorescence lifetime τsample and a ratiometry Rsample obtained by this measurement are used to judge whether or not the test object has the approaching/binding property or the separating property.
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