DIRECT ASSAY OF THIOREDOXIN REDUCTASE ACTIVITY
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United States of America Patent
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Feb 19, 2015
app pub date -
Aug 15, 2014
filing date -
Aug 15, 2013
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Abstract
Provided is a direct method for detecting thioredoxin reductase (TR) activity in test samples. The method can provide a continuous and real-time measurement of TR activity. The method comprises contacting the test sample with NADPH and a diselenide substrate of TR, and then measuring conversion of NADPH to NADP. Also provided are kits for use in the method of direct detection of TR activity.
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