Recombinant cellular iysate system for producing a product of interest

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United States of America Patent

PATENT NO 10036001
SERIAL NO

14542074

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Abstract

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The embodiments described herein pertain to cells, and methods for preparing cells, that can be used as biocatalysts by altering enzymes that compete for a substrate or product of a pathway of interest such that the targeted enzyme is sensitive to a site-specific protease, which protease is expressed but relocated in the cell to a site where it is not in contact with the targeted enzyme in the intact cell. Upon cell lysis, the protease contacts the target enzyme, which is then inactivated by protease cleavage.

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Patent Owner(s)

  • THE BOARD OF TRUSTEES OF THE LELAND STANFORD JUNIOR UNIVERSITY; GREENLIGHT BIOSCIENCES, INC.

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Swartz, James R Menlo Park, US 43 795

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