NUCLEIC ACID AMPLIFICATION METHOD, NUCLEIC ACID EXTRACTION DEVICE, NUCLEIC ACID AMPLIFICATION REACTION CARTRIDGE, AND NUCLEIC ACID AMPLIFICATION REACTION KIT

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United States of America Patent

APP PUB NO 20150099279A1
SERIAL NO

14509278

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Abstract

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A nucleic acid amplification method includes: adsorbing a nucleic acid onto fine particles by mixing a chaotropic substance-containing adsorbent liquid and the fine particles with a nucleic acid-containing sample; washing the fine particles adsorbing the nucleic acid with a first washing liquid; eluting the nucleic acid adsorbed to the fine particles in an eluate; and performing a nucleic acid amplification reaction on the nucleic acid in the eluate. The adsorbent liquid contains an alcohol, and the first washing liquid is acidic. This method can be carried out by a nucleic acid extraction device, a nucleic acid amplification reaction cartridge, and a nucleic acid amplification reaction kit.

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Patent Owner(s)

Patent OwnerAddress
SEIKO EPSON CORPORATIONSHINJUKU-KU TOKYO

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
HANAMURA, Masato Shiojiri, JP 28 17
SAITO, Yuji Shiojiri, JP 213 1710
YAMADA, Kiyohito Shiojiri, JP 3 0

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