Selection in fungi

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United States of America Patent

PATENT NO 9487767
APP PUB NO 20150175981A1
SERIAL NO

14403355

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Abstract

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The present invention relates to methods for constructing a recombinant fungal host cell comprising one or more copies of a polynucleotide construct integrated in its genome, said method comprising transforming a fungal host cell with an integrative polynucleotide construct comprising a first polynucleotide encoding a selectable marker, wherein the first polynucleotide, a 5′ untranslated region thereof and/or a riboswitch operably linked therewith comprises a spliceosomal intron which has 5 nucleotides or less between its branch site and its acceptor site; and a second polynucleotide encoding a polypeptide of interest; as well as suitable polynucleotide constructs, resulting fungal host cells and methods of manufacture.

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Patent Owner(s)

  • NOVOZYMES A/S;NOVOZYMES A/S OF KROGSHOEJVEJ 36

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Inventor(s)

Inventor Name Address # of filed Patents Total Citations
Olsen, Carsten Lillelund Bagsvaerd, DK 6 27

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