METHOD FOR EXTRACTING B-AMYLASES FROM A SOLUBLE FRACTION OF A STARCH PLANT AND IN THE PRESENCE OF A PROTEASE
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United States of America Patent
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Jul 2, 2015
app pub date -
Aug 6, 2013
filing date -
Aug 7, 2012
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Abstract
An improved method for extracting β-amylases from a soluble fraction of a starch plant, includes a step of clarification by microfiltration and a step of concentration and purification by ultrafiltration. This method is characterized in that it uses a protease during the microfiltration step, making it possible to greatly reduce the fouling of the membranes used, which increases the production time before washing. Simultaneously, perfectly clear permeates are obtained, which assists with the subsequent ultrafiltration step and makes it possible to improve the transmission of β-amylase. Finally, the protease has no negative effect on the β-amylase activity.
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