GRAM STAINING METHOD WITH IMPROVED DECOLORIZATION OF THE CRYSTAL VIOLET-IODINE COMPLEX FROM GRAM NEGATIVE BACTERIA
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United States of America Patent
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Issued Date -
Jul 30, 2015
app pub date -
Apr 7, 2015
filing date -
Mar 19, 2012
priority date (Note) -
Abandoned
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Abstract
Provided herein are methods of staining biological material for the purpose of detecting, and in some examples also identifying, microorganisms. Methods of Gram staining bacteria using a slow-acting decolorizing formulation, such as one that includes 1,2-propandiol or ethylene glycol, can be used to extend the time of the decolorizing step, and thus permit automation of the Gram staining method. Also provided are compositions and kits for performing automated Gram staining on microscope slides.
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