METHOD OF OBTAINING CHICKEN EMBRYONIC STEM CELLS
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United States of America Patent
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Issued Date -
Aug 13, 2015
app pub date -
Dec 30, 2014
filing date -
Aug 9, 2006
priority date (Note) -
Abandoned
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Abstract
The present invention concerns a method of culturing embryonic stem (ES) cells of avian, comprising the steps of: a) suspending ES cells originating from the blastoderm disk of fertilized un-incubated avian egg(s) in a basal culture medium supplemented with: insulin-like growth factor-1 (IGF-1) and ciliary neurotrophic factor (CNTF); and animal serum; and optionally, at least one growth factors selected in the group comprising interleukin 6 (II-6), interleukin 6 receptor (II-6R), stem cell factor (SCF), fibroblast growth factor (FGF), leukaemia inhibitory factor (LIF), interleukin 11 (II-11), oncostatin and cardiotrophin; b) seeding the suspension of ES cells obtained in step a) on a layer of feeder cells and further culturing the ES cells for at least between 2 to 10 passages; c) optionally removing at least one growth factors selected SCF, FGF, II-6, II-6R, LIF, oncostatin and cardiotrophin and II-11 from the culture medium; d) further culturing the ES cells in the medium of step c) on a layer of feeder cells.
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